Monoclonal Antibody for studying Ku70 in the Cell Cycle / Checkpoint research area.

Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex.

Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-27538. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (0.25 mg/mL). 2021-04-04 Ku70 plus Ku80 double knockout potently inhibits LPS-induced production of pro-inflammatory cytokines in human macrophages In order to exclude the possible off-target effect of the applied Ku70 and Ku80 shRNAs, we established the CRISPR/Cas9-gene editing methods to complete knockout Ku70 and Ku80.

Ku70

Kontrolluppgiftsblanketter. Det finns ett flertal olika kontrolluppgiftsblanketter i inkomstslaget tjänst: KU10 (lön och förmåner m.m.), KU13 (SINK), KU14 (pensionsgrundande inkomst m.m.), KU16 (sjöinkomst), KU17 (SINK med sjöinkomst), KU18 (pensions- och försäkringsutbetalare) och KU19 (pensions- och försäkringsutbetalare med SINK). Size: 609 amino acids Molecular mass: 69843 Da Quaternary structure: Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Ku70 and Ku80 co-immunoprecipitated with p65-p52 NFκB complex in the nuclei of LPS-treated THP-1 cells. Significantly, LPS-induced NFκB activation was inhibited by Ku70 plus Ku80 double knockdown or DKO. It was however enhanced with Ku70 and Ku80 overexpression. The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway.

ku70 tert : gl1; ku70; tert: images; None available : phenotypes ; Surovtseva YV, Shakirov EV, Vespa L, Osbun N, Song X, Shippen DE(2007) In contrast to the ku70 mutant, which undergo telomerase-dependent expansion to more than twice the normal length in a single generation, the ku70 tert double mutant displays accelerated telomere shortening and a precocious onset of genome stability.

Jun 20, 2012 The Ku heterodimer complex consists of Ku70 and Ku80 and is a very versatile protein that regulates a wide range of important and diverse

loss of expression correlates with decreased survival in gall bladder malignancies patients SMAR1-mediated regulation of repair and apoptosis via a complex crosstalk involving Ku70, HDAC6 and Bax. Single-stranded DNA-dependent ATP-dependent helicase. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair. When associated with KU80, binds to double-stranded telomeric and non-telomeric DNA sequences, but not to single-stranded DNA. Recognition of DNA double‐strand breaks during non‐homologous end joining is carried out by the Ku70–Ku80 protein, a 150 kDa heterodimer that recruits the DNA repair kinase DNA‐dependent protein kinase catalytic subunit (DNA‐PKcs) to the lesion. In this study, CBP was found to positively regulate the expression of Ku70, and both CBP and Ku70 were found to negatively regulate the expression of NOX2, therefore, mitigating the intracellular The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins.

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Diseases associated with XRCC6 include Lupus Erythematosus and Nijmegen Breakage Syndrome.Among its related pathways are Cytosolic sensors of pathogen-associated DNA and DNA damage_NHEJ mechanisms of DSBs repair.Gene Ontology (GO) annotations related to this gene include protein C-terminus binding. ku70 tert : gl1; ku70; tert: images; None available : phenotypes ; Surovtseva YV, Shakirov EV, Vespa L, Osbun N, Song X, Shippen DE(2007) In contrast to the ku70 mutant, which undergo telomerase-dependent expansion to more than twice the normal length in a single generation, the ku70 tert double mutant displays accelerated telomere shortening and a precocious onset of genome stability. Plasmid pEGFP-C1-FLAG-Ku70 from Dr. Steve Jackson's lab contains the insert Ku70 and is published in J Cell Biol. 2013 Jul 29.

Report XRCC6 impacts age-related decline in telomere length but is not associated with longevity. Thus, the Ku70 shRNA cells expressed less Ku70 protein than the Ku70 +/− cells but had a lower, and not the predicted higher, frequency of gene targeting (Tables 1 and 2). This was probably caused by the much slower growth of the Ku70 shRNA cells (F.J.F., unpublished observations), which may have a deleterious effect on gene targeting because rAAV preferentially transduces actively dividing Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. The Ku70‐specific C‐terminal region consists of a 5 kDa SAP domain (Ku70‐SAP), the structure of which was determined both in isolation (Zhang et al, 2001) and as part of the Ku70–Ku80 crystal structure (Walker et al, 2001; Fig 1C). Ku antigen is a complex of Ku70 and Ku80 subunits and plays an important role in not only DNA double-strand breaks (DSB) repair and V(D)J recombination, but also in growth regulation.
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Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96].

Ku70 (also known as XRCC6) is a eukaryotic protein that is involved in the repair of DNA double-strand breaks by non-homologous end-joining [ 1, 2 ] Ku is a heterodimer of approximately 70kDa and 80kDa subunits The Ku70/Ku80 heterodimer has ATP-dependent DNA helicase activity and functions as the DNA-binding regulatory component of DNA-dependent protein kinase (DNA-PK) (6-8). The assembly of the DNA-PK complex at DNA ends is required for nonhomologous end-joining (NHEJ), one mechanism involved in double-stranded DNA break repair and V(D)J recombination (8). KU70 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC),Enzyme-linked Immunosorbent Assay (ELISA) applications.
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Invitrogen Anti-Ku70 Polyclonal, Catalog # PA5-27538. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (Paraffin) (IHC (P)), Immunoprecipitation (IP) and Proximity Ligation Assay (PLA) (PLA) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (0.25 mg/mL).

The two Ku subunits form a basket-shaped structure that threads onto the DNA end. Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V (D)J recombination product formation defects. Immunogen Synthetic peptide corresponding to Human Ku70 aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin.

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Anti-Ku70 Protein Antibody from rabbit; Synonym: X-ray repair cross- complementing protein 6, 5′-deoxyribose-5-phosphate lyase Ku70, 5′-dRP lyase Ku70,

Ku70 and Ku80 make up the Ku70/80 heterodimer. They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes.

2001-03-01

Att royaltyn ska redovisas som tjänst gäller däremot inte om den som fått royaltyn inte ska betala skatt i Sverige och inte heller omfattas av svensk socialförsäkring. I de fallen ska royaltyn alltid redovisas på KU70. Ku70 and Ku80 make up the Ku70/80 heterodimer.

Genom att surfa vidare godkänner du att vi använder kakor. Kom ihåg att KU70 inte behöver lämnas då den som mottar näringsbidraget är en juridisk person. Ruta 252 – Utländskt skatteregistreringsnummer (TIN) Observera! Dessa uppgifter finns inte med i e-tjänsten Lämna kontrolluppgifter. Ku70 and Ku80 make up the Ku70/80 heterodimer. They are encoded by the Xrcc5 and Xrcc6 genes, respectively, and are highly abundant in both prokaryotes and eukaryotes.